Cell Health and Mechanism of Action

Assessment of cell survival and cytotoxic responses

Evaluating cell viability is essential for understanding treatment effects, drug tolerability, and immune cell performance. Redoxis provides robust and high-throughput viability assays to quantify cell health following stimulation, drug treatment, or culture conditions.

What we measure

• Overall cell survival

• Early and late apoptosis

• Necrosis and membrane integrity

• Metabolic activity

Example readouts

• Flow cytometry viability dyes

• Annexin V / PI apoptosis profiling

• MTT, WST-1, or resazurin metabolic assays

Applications

• Dose-finding and tolerability studies

• Preliminary toxicity screening

• Immune activation profiling

• Validation of compound effects in target cells

Evaluation of compound-induced immune toxicity and safety signals

Our toxicity assays quantify immune-related and general cytotoxicity to help predict safety concerns early. These assays support hit-to-lead optimization, selection of safe dosing strategies, and identification of potential off-target effects.

What we measure

• Apoptosis and necrosis

• Inflammatory toxicity (e.g., cytokine storm markers)

• Mitochondrial toxicity

• Immune cell subset sensitivity

Example readouts

• Annexin V / PI or 7-AAD

• Caspase activity

• Cytokine release (IL-6, TNF-α, IL-1β)

• Mitochondrial membrane potential assays

Applications

• Safety profiling of biologics, small molecules, ATMPs

• Off-target immune activation

• Risk assessment for immune-related adverse effects

Quantification of immune cell division and activation

Proliferation is a key indicator of functional immune responses and therapeutic activity. Redoxis offers standardized proliferation assays for T cells, B cells, and other immune subsets using dye dilution and phenotype analysis.

What we measure

• T cell proliferation upon CD3/CD28 or antigen stimulation

• B cell or mixed immune cell proliferation

• Activation state and division index

• Immunomodulatory effects of compounds

Example readouts

• CellTrace™ violet or CFSE dilution

• Flow cytometry activation markers (CD25, CD69)

• Cytokine secretion linked to proliferation

Applications

• Immunomodulatory drug assessment

• Vaccine research

• Autoimmunity and inflammation studies

• Mechanism-of-action profiling

Analysis of signaling pathways and drug effects on phospho-proteins

Phosphorylation assays provide detailed insights into early cell signaling events, kinase activity, and pathway modulation — crucial for targeted therapies and mechanistic studies.

What we measure

• Kinase activation

• Upstream and downstream signaling events

• Inhibition or activation of specific pathways

Example readouts

• Phospho-specific flow cytometry

• Western blot for phospho-proteins

• ELISA-based phosphorylation assays

Applications

• Kinase inhibitor development

• Mechanism-of-action studies

• Pathway validation

• Cell signaling research in immunology and oncology