Reporter and Transfection Assays

Sensitive tools to evaluate pathway activation, transcriptional control, and drug effects

Reporter gene assays allow precise tracking of gene expression or signaling pathway activity using fluorescent or luminescent readouts. These assays are ideal for targeted analysis of upstream transcription factor activity or downstream signal transduction.

What we measure

• NF-κB activation

• AP-1 or STAT pathway dynamics

• Interferon or cytokine response pathways

• Promoter activity of genes of interest

• Pathway inhibition by kinase or receptor-targeting drugs

Readouts

• Luminescence or fluorescence intensity

• Dose-response and time-course dynamics

• Reporter normalization for high precision

Reporter systems available

• Luciferase-based reporters

• Fluorescent reporters (GFP, RFP variants)

• Dual-reporter systems for internal normalization

• Custom promoters (upon request)

Rapid evaluation of gene function, target modulation, or signaling effects

Transient transfection enables short-term, flexible expression of genes, constructs, or reporter systems. This approach is ideal for early screening and for evaluating immediate cellular responses to genetic or pharmacological manipulation.

Capabilities

• High-efficiency transfection of cell lines or primary cells (where applicable)

• Expression of target genes, transcription factors, or pathway components

• Co-transfection of reporter constructs for functional readouts

• siRNA/shRNA-based knockdown studies (optional)

What it enables

• Rapid gene expression studies

• Functional characterization of pathways

• Screening of gene–drug interactions

• Mechanistic dissection of immune-modulatory responses

Applications

• Target validation

• Synthetic biology and engineered cell models

• Early mechanistic insights into immune signaling

Long-term expression systems for reproducible and consistent readouts

Stable transfection provides continuous expression of genes or reporters, ensuring robust and reproducible assays suitable for extended experiments or multiple study cycles.

Capabilities

• Generation of stable cell lines expressing reporter constructs

• Antibiotic selection and clonality validation

• Expression of therapeutic targets, receptors, or engineered pathways

What it enables

• Longitudinal functional assays

• Stable expression of reporter or effector genes

• Mechanistic studies with low experimental variation

• Batch-to-batch reproducibility across studies

Applications

• Drug screening and regulatory pathway evaluation

• Long-term studies of immune activation or suppression

• Continuous assessment of pharmacological pathway modulation

Tailored systems based on your biological pathway or therapeutic target

Redoxis provides custom assay development aligned with the biology of your target pathway.

Examples of custom systems

• Pathway-specific promoters (e.g., interferon response element)

• Inflammatory signaling reporters (e.g., NF-κB, IRF transcription factors)

• Cell type–specific expression systems (macrophages, T cells, microglia)

• Drug-induced transcriptional activation reporters

What we offer

• Assay design & optimization

• Validation of reporter dynamics

• Dose-response and EC50/IC50 characterization

• Integration with downstream molecular readouts