Molecular and Biomarker applications

Quantitative measurement of soluble immune mediators

Cytokines and chemokines are key biomarkers of immune activation and regulation. Redoxis provides sensitive, multiplexed, and highly reproducible detection of soluble mediators in serum, plasma, tissue homogenates, culture supernatants, and sorted immune cell populations.

What we measure

• Pro-inflammatory cytokines (e.g., IL-1β, IL-6, TNF-α, IFN-γ)

• Anti-inflammatory mediators (e.g., IL-10, TGF-β)

• Chemokines relevant to immune recruitment

• Toxicity-related biomarkers

Technologies

• Luminex multiplex analysis

• ELISA

• FluoroSpot / ELISpot (single-cell cytokine secretion)

Applications

• Mechanism-of-action profiling

• Biomarker discovery

• Cytokine storm and toxicity risk assessment

• Immune monitoring in in vivo studies

High-sensitivity analysis of gene regulation and pathway modulation

Gene expression analysis is a cornerstone for understanding molecular responses and transcriptional regulation. Redoxis offers complete workflows — from tissue homogenization to data-ready results — using qPCR and droplet digital PCR (ddPCR).

Capabilities

• Tissue homogenization and RNA extraction

• Reverse transcription and amplification

• Absolute quantification via ddPCR

• Targeted pathway analysis via qPCR

What we measure

• Transcription factors (e.g., T-bet, RORγt, FoxP3, GATA3)

• Cytokine and chemokine gene expression

• Inflammasome-related genes (e.g., NLRP3, IL1B, CASP1)

• Signaling pathway genes (NF-κB, JAK-STAT, MAPK, etc.)

Applications

• Mechanism-of-action studies

• Pathway modulation by therapeutics

• In vivo tissue profiling

• Validation of flow cytometry or protein-level findings

Unbiased or targeted analysis of gene expression at bulk or single-cell level

Redoxis offers both bulk RNA-seq and single-cell RNA-seq to provide high-resolution molecular insights.

Single-Cell RNA-seq

What it enables

• Cell-type-specific transcriptional profiling

• Characterization of cellular heterogeneity

• Identification of rare or treatment-responsive subsets

• Immune repertoire changes (optional)

Ideal for:

• CNS inflammation, MS, IBD, oncology

• Heterogeneous immune responses

• ATMP and biologics characterization

Bulk RNA-seq

What it enables

• Whole-transcriptome profiling

• Differential gene expression (DGE) analyses

• Pathway and network analysis

• Low-input or sorted-cell applications

Ideal for:

• Tissue samples, cultured cells, purified populations

• Biomarker discovery

• Mechanistic pathway interrogation

Quantifying protein levels, phosphorylation, and pathway activation

Western blotting provides detailed protein-level confirmation of molecular mechanisms.

What we measure

• Total protein expression

• Phospho-protein levels (signaling activation)

• Biomarker validation

• Pathway-specific targets (NF-κB, ERK, STAT, AKT, etc.)

Applications

• Kinase inhibitor profiling

• Validation of gene expression changes

• Mechanistic studies in inflammation and oncology

Single-cell cytokine secretion profiling

These assays quantify the frequency of cytokine-secreting cells at the single-cell level.

ELISpot

• Single cytokine per well

• High sensitivity

FluoroSpot

• Multiplex detection (two or more cytokines simultaneously)

• Ideal for Th1/Th2/Th17 balance or polyfunctionality

Applications

• Vaccine research

• T cell memory studies

• Autoimmunity and tolerance assessments

• Functional immunology

Tissue-level evaluation of morphology, infiltration, and biomarkers

Histology supports interpretation of in vivo and ex vivo findings by revealing tissue integrity, pathology, or immune infiltration.

Capabilities

• Tissue processing and sectioning

• H&E staining

• IHC for cell types (e.g., CD3, F4/80) or biomarkers

• Imaging and semi-quantitative scoring

Applications

• In vivo model characterization

• Mechanistic localization of immune responses

• Tissue-level toxicity assessment

• Biomarker validation

Hematological biomarker analysis and immune cell composition

Sysmex analysis provides quantitative assessment of:

• RBC, WBC, platelet profiles

• Differential leukocyte counts

• Immune cell dynamics during disease

• Hematological safety markers

Useful for:

• In vivo model characterization

• Toxicity studies

• Treatment-response monitoring