B and T cell biology

Investigating Th1, Th2, Th17, Treg, and hybrid T cell subsets

T cell differentiation assays model how naïve CD4⁺ T cells polarize into defined effector subsets under controlled cytokine environments.

What we measure

• Th1, Th2, Th17, Th22, Th1/Th17, Treg, Th9 differentiation

• Chemokine receptor patterns (CXCR3, CCR4, CCR6, CCR10)

• Transcription factors (T-bet, GATA3, RORγt, FoxP3)

• Cytokine secretion profiles

Readouts

• Multicolor flow cytometry (standardized gating)

• Luminex or ELISA cytokine quantification

• qPCR for lineage-associated genes

Applications

• Mechanism-of-action in autoimmune diseases

• Evaluation of biologics and small molecules

• T cell–directed immunomodulation

• Treg/Th17 balance studies

Generating and characterizing inducible regulatory T cells

Regulatory T cells (Tregs) are essential for immune tolerance and controlling inflammation. This assay evaluates a compound’s ability to promote or inhibit Treg generation.

What we measure

• FoxP3 expression

• IL-10 and TGF-β secretion

• Treg-associated surface markers

• Stability of induced Tregs

Applications

• Tolerance induction therapies

• Autoimmunity research

• Inflammation control mechanisms

Functional assessment of Treg-mediated immune regulation

This assay quantifies the ability of Tregs to suppress effector T cell proliferation and cytokine secretion.

What we measure

• Suppression of CD4⁺ or CD8⁺ Teff proliferation

• Reduction in pro-inflammatory cytokines

• Treg:Teff ratio-dependent suppression curves

Readouts

• CellTrace™ dilution (proliferation index)

• IFN-γ, IL-2, TNF-α secretion

• Flow cytometry for activation markers

Applications

• Drug effects on immune regulation

• Autoimmunity and transplantation

• Functional potency of Treg-inducing compounds

Alloreactivity and T cell activation in a physiologically relevant system

MLR assays model how T cells respond to foreign antigens presented by allogeneic dendritic cells or monocytes.

What we measure

• Alloreactive T cell proliferation

• Cytokine production (IFN-γ, IL-17, IL-2)

• Immune suppression or activation by candidate therapeutics

Applications

• Transplantation and graft rejection modeling

• Immunomodulatory screening

• Mixed-population immune activation analysis

Antigen-specific memory T cell responses

Recall assays evaluate how memory T cells respond when re-exposed to a known antigen.

What we measure

• Antigen-specific T cell proliferation

• Activation marker expression

• Effector cytokine production

• Memory subset profiles

Antigens

• Viral antigens (CMV, EBV, influenza)

• Autoantigens (project-specific)

• Tumor antigens (upon request)

Applications

• Vaccine evaluation

• Chronic inflammation research

• Monitoring long-term immune responses

Immediate activation signatures and functional potency

Activation assays measure early and intermediate activation markers following stimulation.

Markers measured

• CD69, CD25, CD71

• PD-1, ICOS, CTLA-4 (optional)

• Cytokine release and proliferation

Applications

• Mechanism-of-action profiling

• Immunotherapy and checkpoint inhibitor evaluation

• Early T cell activation dynamics

Comprehensive multiparameter immunophenotyping

Using standardized and customizable flow cytometry panels, we analyze:

• CD4⁺ and CD8⁺ T cell subsets

• Memory populations (naïve, central memory, effector memory, TEMRA)

• Helper subsets (Th1, Th2, Th17, Th22, Th1/Th17, etc.)

• Regulatory T cells (FoxP3⁺CD25⁺CD127low)

• Activation/exhaustion markers

Applications

• Deep immune profiling

• Translational biomarker discovery

• Integration with in vivo or clinical samples