Anti-GBM Nephritis – Preclinical Model of Immune-Mediated Glomerulonephritis

Anti-GBM (anti–glomerular basement membrane) nephritis is a widely used preclinical model for studying immune-mediated kidney inflammation and crescentic glomerulonephritis. The model mimics features of rapidly progressive glomerulonephritis (RPGN) and Goodpasture’s syndrome, which are characterized by autoantibody deposition in the glomerular basement membrane, complement activation, and immune cell infiltration leading to renal damage and loss of function.

At Redoxis, we offer the anti-GBM nephritis model in mice, induced by passive immunization with nephrotoxic serum containing anti-GBM antibodies. This leads to acute renal inflammation, proteinuria, and histological changes such as crescent formation and glomerular necrosis.

This model is induced by intravenous or intraperitoneal injection of heterologous anti-GBM serum (e.g., rabbit anti-mouse GBM) in susceptible mouse strains such as C57BL/6. Disease onset is rapid, and kidney injury develops within days of antibody administration.

Inflammation is driven by immune complex deposition, complement activation, and leukocyte recruitment, particularly neutrophils and macrophages. This model is well suited for evaluating interventions targeting humoral immunity, complement pathways, or innate effector mechanisms.

Key Features:

  • Rapid-onset glomerulonephritis induced by anti-GBM antibody injection
  • Proteinuria and elevated serum creatinine as markers of kidney injury
  • Histological hallmarks: crescent formation, leukocyte infiltration, glomerular necrosis
  • Involves both humoral and innate immune mechanisms
  • Suitable for testing complement inhibitors, anti-inflammatory agents, and renal-protective therapies
  • Compatible with histology, kidney function markers, and cytokine profiling

Redoxis supports a wide range of readouts in this model, including urine protein analysis, serum creatinine, kidney histopathology, and flow cytometry of renal immune infiltrates. The anti-GBM nephritis model provides a robust and translationally relevant system for assessing treatments in severe, antibody-driven kidney inflammation.

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Inquiries? Questions?

Nina Woodworth

COO